890 Does insulin-like growth factor (IGF-1) gene polymorphism affect acne?

Introduction: Acne vulgaris is the most typical disorder affecting adolescents. Heritability 80% in first-degree relatives. Insulin-like growth factor (IGF-1) structurally and functionally related to insulin. It consists of about 195 amino acids located on cytogenetic band 12q23.2. Studies have shown an association between IGF-1 signaling which plays a role acne by levels androgen metabolism. Variability shows genetic regulation. There functional relationship IGF (CA19) polymorphism levels. varied incidences needs further probing. Objectives: To determine gene frequency Pakistani population compare this cases controls. Methodology: Case-control study Dermatology Department, Mayo Hospital, Lahore, Pakistan. Two hundred seventy patients 80 age gender-matched controls were recruited non-probability purposive sampling. Blood was drawn after overnight fast for genomic DNA extraction PCR determined polymorphism. Forward primers labeled with FAM (6-carboxyfluorescein) analyze product. The polymorphisms compared Quantitative variables expressed as median percentiles data non-normal. Comparisons done Mann-Whitney U test correlations Spearman’s correlation. A p-value ≤ 0.05 considered statistically significant. Results: differed significantly I87/x frequent both In cases, (187/187) homozygous allele; controls, it 187 heterozygous allele (187/x). some higher Gene associated serum These findings will help establishing endocrine basis can be used pharmacogenomics.